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Deep eutectic system enhanced oat protein extraction.
Wuxia Ge1, Zhixin Xiao1, Xinru Ding2
1Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, China.
Journal of Food Science
|January 19, 2025
Summary
This study introduces a green deep eutectic system (DESys) method for extracting oat proteins (OPs). Using choline chloride (ChCl) significantly improved protein yield and offered tunable properties, presenting an eco-friendly alternative to traditional methods.
Area of Science:
- Food Science
- Green Chemistry
- Biochemistry
Background:
- Oat proteins (OPs) are valuable plant-based protein sources.
- Traditional alkali solution acid precipitation (ASAP) for OPs causes pollution and protein denaturation.
- Deep eutectic solvents (DESs) offer a potential alternative for protein extraction.
Purpose of the Study:
- To investigate deep eutectic solvents (DESs) and deep eutectic systems (DESys) for efficient and eco-friendly oat protein extraction.
- To compare the protein recovery yield and physicochemical properties of OPs extracted using DESys, DES, and ASAP methods.
- To identify an optimal DESys-based method considering toxicity and yield.
Main Methods:
- Extraction of oat proteins using deep eutectic solvents (DESs) and deep eutectic systems (DESys).
- Systematic investigation of extraction conditions, including choline chloride (ChCl) as a hydrogen bond acceptor (HBA).
- Comparison of protein recovery yield and physicochemical properties (molecular structure, amino acid composition, thermal properties) with traditional ASAP method.
Main Results:
- The DESys-based method with ChCl as HBA significantly increased protein recovery yield compared to ASAP and DES methods.
- Physicochemical properties of extracted OPs varied across methods, indicating potential for property adjustment.
- The DESys-based method demonstrated lower toxicity and higher protein recovery, making it a suitable alternative.
Conclusions:
- Deep eutectic system (DESys) extraction with choline chloride (ChCl) is a green and efficient method for oat protein isolation.
- This approach minimizes environmental impact and offers tunable protein properties.
- The study provides insights for developing novel DES/DESys-based extraction strategies for biomolecules.
