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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
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Deep eutectic system enhanced oat protein extraction.

Wuxia Ge1, Zhixin Xiao1, Xinru Ding2

  • 1Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, China.

Journal of Food Science
|January 19, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a green deep eutectic system (DESys) method for extracting oat proteins (OPs). Using choline chloride (ChCl) significantly improved protein yield and offered tunable properties, presenting an eco-friendly alternative to traditional methods.

Keywords:
deep eutectic solventdeep eutectic systemionic liquidoat proteinprotein extraction

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Area of Science:

  • Food Science
  • Green Chemistry
  • Biochemistry

Background:

  • Oat proteins (OPs) are valuable plant-based protein sources.
  • Traditional alkali solution acid precipitation (ASAP) for OPs causes pollution and protein denaturation.
  • Deep eutectic solvents (DESs) offer a potential alternative for protein extraction.

Purpose of the Study:

  • To investigate deep eutectic solvents (DESs) and deep eutectic systems (DESys) for efficient and eco-friendly oat protein extraction.
  • To compare the protein recovery yield and physicochemical properties of OPs extracted using DESys, DES, and ASAP methods.
  • To identify an optimal DESys-based method considering toxicity and yield.

Main Methods:

  • Extraction of oat proteins using deep eutectic solvents (DESs) and deep eutectic systems (DESys).
  • Systematic investigation of extraction conditions, including choline chloride (ChCl) as a hydrogen bond acceptor (HBA).
  • Comparison of protein recovery yield and physicochemical properties (molecular structure, amino acid composition, thermal properties) with traditional ASAP method.

Main Results:

  • The DESys-based method with ChCl as HBA significantly increased protein recovery yield compared to ASAP and DES methods.
  • Physicochemical properties of extracted OPs varied across methods, indicating potential for property adjustment.
  • The DESys-based method demonstrated lower toxicity and higher protein recovery, making it a suitable alternative.

Conclusions:

  • Deep eutectic system (DESys) extraction with choline chloride (ChCl) is a green and efficient method for oat protein isolation.
  • This approach minimizes environmental impact and offers tunable protein properties.
  • The study provides insights for developing novel DES/DESys-based extraction strategies for biomolecules.