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Stevioside as a potential therapeutic agent: inhibiting cell growth and regulating epithelial-mesenchymal transitions
Vishnu Priya Veeraraghavan1, Selvaraj Jayaraman1, Maria Maddalena Marrapodi2
1Center of Molecular Medicine and Diagnostics (COMManD), Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Background:
Oral cancer is a predominant and aggressive form of head and neck cancer with limited treatment options. Stevioside, a naturally occurring biocompatible compound, has gained attention for its potential therapeutic properties, although its molecular mechanistic role in OSCC merely understood. This study aims to elucidate the impact of stevioside on OSCC cells, focusing on its inhibitory effects on cell proliferation and epithelial-mesenchymal transitions (EMT).
Methods:
KB cells, representative of OSCC, were subjected to stevioside treatment in a time-dependent manner. The findings obtained from the MTT assay revealed a notable suppression of KB cell growth following 48 hours of treatment with stevioside.
Results:
The IC
Conclusions:
In conclusion, this study sheds light on the significant role of stevioside in oral cancer. The observed inhibition of KB cell proliferation and its pronounced impact on EMT-related gene expression indicate the potential of stevioside as a promising therapeutic agent in the context of OSCC. The multifaceted effects of stevioside on OSCC cells present an exciting avenue for advancing our understanding of this devastating disease and the development of innovative therapeutic strategies.
Insights
Stevioside significantly inhibits oral cancer cell proliferation and impacts epithelial-mesenchymal transition (EMT) gene expression. This natural compound shows promise as a therapeutic agent for oral squamous cell carcinoma (OSCC).
Area of Science:
- Oncology
- Natural Product Chemistry
- Molecular Biology
Background:
- Oral cancer, specifically oral squamous cell carcinoma (OSCC), is aggressive with limited therapeutic options.
- Stevioside, a natural compound, exhibits potential therapeutic properties, but its mechanism in OSCC is not fully understood.
- This study investigates stevioside's effects on OSCC cell proliferation and epithelial-mesenchymal transitions (EMT).
Purpose of the Study:
- To elucidate the impact of stevioside on oral squamous cell carcinoma (OSCC) cells.
- To evaluate stevioside's inhibitory effects on cell proliferation and EMT in OSCC.
Main Methods:
- KB cells (OSCC model) were treated with stevioside in a time-dependent manner.
- MTT assay was used to assess cell viability and growth inhibition.
- Gene expression analysis (RT-PCR) and molecular docking were employed to study EMT-related genes.
Main Results:
- Stevioside treatment significantly suppressed KB cell growth after 48 hours.
- The IC50 value for stevioside's inhibitory effect was determined to be 110.54 µM.
- Analysis revealed stevioside's impact on the expression of EMT-related genes (ECADH, SNAIL1, SLUG, VIM).
Conclusions:
- Stevioside demonstrates significant inhibitory effects on oral cancer cell proliferation.
- The compound markedly influences EMT-related gene expression in OSCC.
- Stevioside emerges as a potential therapeutic agent for oral squamous cell carcinoma, warranting further investigation.
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