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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

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Characterizing the extractable proteins from tomato leaves - A proteomics study.

Marietheres Kleuter¹, Yafei Yu², Lukas Verdegaal¹

¹Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, the Netherlands.

Food Chemistry: X

|January 20, 2025

Summary

This summary is machine-generated.

Tomato leaves offer plant-based protein, but yields decrease as plants mature. This is due to functional shifts and inhibitory molecules, impacting protein extraction efficiency.

Keywords:

Agricultural by-products Plant development Protein extraction Proteomics analysis Soluble proteins Tomato – Solanum lycopersicum

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Last Updated: Jun 1, 2025

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Area of Science:

Agricultural Science
Plant Biochemistry
Proteomics

Background:

Agricultural by-products, like tomato leaves, are explored for plant-based protein extraction.
Protein extractability from tomato leaves declines in later developmental stages, limiting industrial application.

Purpose of the Study:

To investigate the factors affecting protein extractability in tomato leaves across different developmental stages.
To identify molecular mechanisms behind reduced protein yields in mature tomato plants.

Main Methods:

Comprehensive proteomics analysis was conducted on tomato leaves from four distinct developmental stages: vegetative, flowering, fruit-forming, and mature-fruit.
Leaf protein profiles were analyzed to understand changes in protein synthesis, degradation, and functional roles.

Main Results:

Reduced protein extractability in later stages correlates with a shift from anabolic to catabolic leaf functions and increased stress responses.
Decreased protein synthesis and elevated protein degradation contribute to a lower soluble protein fraction in mature leaves.
Inhibitory molecules present in later-stage leaves impede efficient soluble protein extraction.

Conclusions:

Breeding strategies should focus on increasing soluble protein content in tomato leaves.
Reducing the concentration of inhibitory molecules in tomato leaves can enhance protein extraction yields.
Optimizing tomato plant development stages for protein extraction is crucial for industrial viability.