Window into the Brain: In Vivo Multiphoton Imaging

Decoding the principles underlying neuronal information processing necessitates the emergence of techniques and methodologies to monitor multiscale brain networks in behaving animals over long periods of time. Novel advances in biophotonics, specifically progress in multiphoton microscopy, combined with the development of optical indicators for neuronal activity have provided the possibility to concurrently track brain functions at scales ranging from individual neurons to thousands of neurons across connected brain regions. This Review presents state-of-the-art multiphoton imaging modalities and optical indicators for in vivo brain imaging, highlighting recent advancements and current challenges in the field.