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Related Experiment Video

Updated: Jun 1, 2025

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

Synaptoneurosome Preparation from C57BL/6 Striata.

Emma Puighermanal^1,2,3, Anne Biever^1,2,3, Emmanuel Valjent^1,2,3

¹CNRS, UMR5203, Institut de Génomique Fonctionnelle, Montpellier, France.

|January 20, 2025

Summary

This summary is machine-generated.

Synaptic plasticity relies on local mRNA translation, regulated by protein phosphorylation. This study details a method to analyze synaptic phospho-proteins in mouse striatal synaptoneurosomes, aiding research into synaptic remodeling.

Keywords:

Imaris Microglia brain cell phenotype morphology neuroinflammation

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Last Updated: Jun 1, 2025

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

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Published on: September 14, 2022

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

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Published on: June 8, 2018

Area of Science:

Neuroscience
Molecular Biology
Cell Biology

Background:

Activity-dependent local mRNA translation is crucial for synaptic plasticity, enabling structural and functional remodeling.
Protein phosphorylation of translational machinery components, including initiation factors and ribosomal proteins, tightly controls this process.

Purpose of the Study:

To describe a reproducible method for preparing striatal synaptoneurosomes from adult mice.
To enable quantification of synaptic phospho-proteins within these isolated synaptoneurosomes.

Main Methods:

Preparation of striatal synaptoneurosomes from adult mice.
Isolation of synaptoneurosomes containing both pre- and postsynaptic elements.
Quantification of synaptic phospho-protein levels.

Main Results:

The protocol successfully yields synaptoneurosomes suitable for phospho-protein analysis.
The method allows for the assessment of phosphorylation states within the synaptic compartment.

Conclusions:

This protocol provides a valuable tool for investigating the role of phosphorylation in activity-dependent mRNA translation at synapses.
Understanding synaptic phospho-protein regulation is key to elucidating mechanisms of synaptic plasticity and function.