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Updated: Jun 1, 2025

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
Published on: March 12, 2020
Unlocking hidden treasures: the evolution of high-throughput mass spectrometry in screening for cryptic natural
Brett C Covington1, Mohammad R Seyedsayamdost1,2
1Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA. mrseyed@princeton.edu.
Abstract:
Covering: 1994 to 2024Historically, microbial natural product discovery has been predominantly guided by biological activity from crude microbial extracts with metabolite characterization proceeding one molecule at a time. Despite decades of bioactivity-guided isolations, genomic evidence now suggests that we have only accessed a small fraction of the total natural product potential from microorganisms and that the products of the vast majority of biosynthetic pathways remain to be identified. Here we describe recent advancements that have enabled high-throughput mass spectrometry and comparative metabolomics, which in turn facilitate high-throughput natural product discovery. These advancement promise to fully unlock the reservoir of microbial natural products.
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