Related Experiment Video
Updated: Jun 1, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
Synthesis, Photophysical Properties and Systematic Evaluation of New Class Fluorescein Based Derivative with Organic
M Sucheta1, A G Pramod1, C G Renuka2
1Department of Physics, Jnanabharathi, Bangalore University, Bengaluru, 560056, Karnataka, India.
Abstract:
In this report the photophysical property of newly synthesized fluorescein based derivative 2-(5-((2,4-dichlorophenyl)diazenyl)-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid has studied by spectroscopic and theoretical that is by Density Functional Theory technique. The structural and functional group of the synthesized molecule was confirmed by nuclear magnetic resonance and fourier transform infrared spectroscopy technique, and from the result so far obtained has been confirmed that molecule has a stable structure and confirmed the presence the functional groups present in the sample. The optical properties of the molecule are studied using the spectroscopic technique and it has revealed the solute-solvent interaction behaviour of the molecule and it has been observed that the bathochromic shift was of about 5 nm, from the fluorescence measurement it has revealed that the emission has been observed at green region and from the power spectra it has been confirmed the same. The DFT study has been derived for the synthesized molecule the optimized structure, electrostatic potential maps, theoretical energy band gap and HOMO and LUMO visualizations are derived from the Gaussian 09W software and it has been found that the bandgap energy is found to be 2.607 eV for benzene solvent and 3.081 eV in methanol solvent, the electrophilic and nucleophilic sites exhibited at the substitution group of the molecule. The outcomes of the study clearly endorse the synthesized fluorescein derivative suitable for photonic/optoelectronic applications.
More Related Videos
Related Concept Videos
Variables Affecting Phosphorescence and Fluorescence
Photoluminescence: Applications
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Fluorescence and Phosphorescence: Instrumentation
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectroscopy: Molecular Electronic Transitions

