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Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

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Related Experiment Video

Updated: Jun 1, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Methylation status of selected genes in non-small cell lung carcinoma - current knowledge and future perspectives.

Adam Put^1,2, Katerina Smesny Trtkova^1,2,3, Jozef Skarda^1,2,3

¹Department of Clinical and Molecular Pathology and Medical Genetics, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic.

|January 20, 2025

Summary

DNA methylation patterns in key genes are altered in non-small cell lung cancer (NSCLC) subtypes. Analyzing these epigenetic changes, particularly in large-cell neuroendocrine carcinoma (LCNEC), can improve NSCLC diagnosis and treatment.

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Last Updated: Jun 1, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

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Published on: October 31, 2016

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Area of Science:

Oncology
Epigenetics
Molecular Biology

Background:

DNA methylation is a crucial epigenetic modification implicated in cancer initiation and progression.
Non-small cell lung cancer (NSCLC) encompasses various histological subtypes with distinct biological behaviors.
Large-cell neuroendocrine carcinoma (LCNEC) is a rare but aggressive subtype of NSCLC.

Purpose of the Study:

To compare promoter methylation status of selected genes across NSCLC histological subtypes.
To investigate the potential of DNA methylation as a biomarker for NSCLC diagnosis, prognosis, and therapy.
To explore the role of LCNEC as a potential transitional entity between NSCLC and small-cell lung carcinoma.

Main Methods:

Comprehensive literature search of PubMed database up to August 17, 2024.
Review of 75 studies focusing on promoter methylation of key genes (e.g., APC, BRCA1, CDH1, CDKN2A, RASSF1) in NSCLC.
Analysis of mutational profiles (TP53, RB1) and gene expression patterns (ASCL1, DLL3) in conjunction with methylation data.

Main Results:

Significant differences in promoter methylation patterns were observed across NSCLC subtypes.
LCNEC exhibited unique methylation profiles, suggesting a potential link to small-cell lung carcinoma.
Selected methylated genes showed potential for diagnostic and prognostic applications in NSCLC.

Conclusions:

Promoter methylation analysis of specific genes offers valuable insights into NSCLC heterogeneity.
Epigenetic profiling, especially in LCNEC, may enhance diagnostic accuracy and guide personalized treatment strategies for NSCLC patients.
LCNEC may represent a distinct biological entity bridging NSCLC and SCLC.