Guidelines for animal models of endurance and resistance exercise

This mini-review details the guideline for implementing the most common exercise patterns in small laboratory rodents (mice/rats) and the advantages and disadvantages of each, in ways that are comparable to humans. Also, criteria for targeted selection and control of workload and intensity of activity are proposed in different exercise programs.

As an available and low-cost intervention in physiological, biochemical and cellular-molecular assessments, different exercise programs can be effective in the prevention/treatment of many skeletal-structural, behavioral and neurodegenerative disorders. Exercise tolerance/intolerance is an indicator of the complex function of the physiological, metabolic, neuromuscular, cardiovascular and respiratory systems, and in this sense, animal models of exercise are of interest to researchers by creating a controllable and precise environment.

Considering the different species of laboratory animals and various exercise paradigms, selecting the type, intensity and duration of the program in an optimal manner is a difficult task, especially in conditions such as old age or illness, and if necessary, existing research tools and protocols should be reviewed. In fact, one of the most attractive applications of exercise models is the discovery of preventive/therapeutic strategies for many disorders, which necessitates more knowledge about exercise protocols.

Animal models of endurance/resistance exercise on land/water make it possible to evaluate physiological/pathological conditions. However, to obtain optimal and reproducible results in human samples, the effectiveness of anesthetic drugs, surgical procedures, and the stress caused by exercise tools and equipment must be carefully controlled.