Related Experiment Video

Updated: Jun 1, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

35.7K

NKD2 as a Mediator of IFIX Antioncogene-Induced Wnt Signalling and Epithelial-Mesenchymal Transition in Human OSCC

Shan Wang1, Haixia Fan2, Jie Bai3

  • 1Department of Oral Pathology, School of Stomatology, Hainan Medical University, Haikou, P. R. China.

Journal of Cellular and Molecular Medicine
|January 20, 2025
PubMed

Insights

Human interferon-inducible protein X (IFIX) inhibits oral cancer progression by suppressing epithelial-mesenchymal transition (EMT) via the NKD2-Wnt signaling pathway. This discovery offers new therapeutic targets for oral squamous cell carcinoma (OSCC).

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • The human interferon-inducible protein X (IFIX) isoform plays a role in cytoskeleton stability and inhibiting epithelial-mesenchymal transition (EMT).
  • The precise mechanisms of IFIX in oncogenesis, particularly in oral squamous cell carcinoma (OSCC), remain unclear.

Purpose of the Study:

  • To investigate the effects of IFIX overexpression and knockdown on OSCC cells.
  • To elucidate the molecular pathways, including Wnt signaling and NKD2, involved in IFIX-mediated oncogenesis.

Main Methods:

  • Utilized CAL-27 and SCC-25 OSCC cell lines for IFIX overexpression and knockdown experiments.
  • Employed quantitative reverse-transcription PCR and Western blotting to analyze gene and protein expression.
  • Investigated the roles of E-cadherin, N-cadherin, vimentin, Snail, Wnt signaling components, and NKD2.

Main Results:

  • IFIX modulation significantly altered the expression of EMT markers (E-cadherin, N-cadherin, vimentin, Snail) and Wnt signaling components.
  • A strong positive correlation was observed between IFIX and NKD2 expression, with both showing modulated expression upon IFIX manipulation.
  • NKD2 silencing mimicked IFIX knockdown effects and reversed IFIX overexpression's anticarcinogenic phenotype, impacting cell proliferation, invasion, and migration.

Conclusions:

  • IFIX exerts antioncogenic effects in OSCC by inhibiting EMT through the NKD2-mediated suppression of Wnt signaling.
  • These findings reveal a novel mechanism for IFIX's role in restricting cancer progression and highlight NKD2 as a key mediator.
Keywords:
EMTIFIXNKD2OSCCWnt

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.6K
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion09:23

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Published on: April 2, 2020

6.4K

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.2K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K