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Updated: Jun 1, 2025
09:13
Quantitative Immunofluorescence to Measure Global Localized Translation
Published on: August 22, 2017
9.8K
Sequencing technologies to measure translation in single cells
Michael VanInsberghe1,2,3, Alexander van Oudenaarden4,5,6
1Oncode Institute, Utrecht, the Netherlands. m.vaninsberghe@hubrecht.eu.
Nature Reviews. Molecular Cell Biology
|January 20, 2025
Summary
New single-cell sequencing technologies now allow researchers to measure cellular translation, overcoming previous limitations in studying complex tissues and dynamic processes. This advances our understanding of protein synthesis regulation at an unprecedented resolution.
Area of Science:
- Molecular Biology
- Genomics
- Cellular Biology
Background:
- Cellular translation is a highly energy-intensive and tightly regulated process.
- Genome-wide methods have advanced the study of protein synthesis regulation.
- Previous technological limitations hindered the analysis of translation in complex biological systems.
Purpose of the Study:
- To review recent advancements in single-cell sequencing technologies for measuring translation.
- To highlight methods enabling translation analysis at single-cell resolution.
- To discuss the implications for studying gene expression regulation.
Main Methods:
- Ribosome profiling
- Ribosome affinity purification
- Spatial translatome methods
- Single-cell sequencing adaptations
Main Results:
- Single-cell resolution measurements of translation are now achievable.
- New techniques overcome limitations of previous genome-wide methods.
- Enables detailed study of translation in rare cell types and dynamic processes.
Conclusions:
- Single-cell translation measurements offer unprecedented insights into gene expression.
- Technological progress is key to understanding complex cellular regulation.
- These methods are crucial for advancing research in multicellular tissues and dynamic cellular events.
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