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Identification and validation of five ferroptosis-related molecular signatures in keloids based on multiple transcriptome data analysis

  • 0Department of Plastic Surgery, Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou, China.
Frontiers in Molecular Biosciences +

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January 21, 2025

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January 21, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study identifies key ferroptosis-related genes involved in keloid formation, offering potential new biomarkers and therapeutic targets for this common skin disorder.

Area Of Science

  • Dermatology
  • Molecular Biology
  • Genetics

Background

  • Keloids are characterized by excessive fibrous tissue growth.
  • Ferroptosis, a regulated cell death pathway, is implicated in fibrosis but its role in keloids is unclear.

Purpose Of The Study

  • To identify key ferroptosis-related genes in keloid formation.
  • To explore their potential as biomarkers or therapeutic targets.

Main Methods

  • Analysis of NCBI GEO datasets (GSE145725, GSE7890, GSE44270, GSE181316).
  • Identification of differentially expressed and ferroptosis-related genes using LASSO regression.
  • Validation via qRT-PCR and Western blot.

Main Results

  • Identified 471 differentially expressed genes; selected five key ferroptosis-related genes.
  • Two genes were upregulated, three downregulated in keloid tissue.
  • These genes are involved in fibrotic processes, confirmed by validation.

Conclusions

  • Novel insights into ferroptosis mechanisms in keloid pathogenesis.
  • Identified ferroptosis genes may serve as potential keloid biomarkers or therapeutic targets.

Keywords: