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Carbonic Anhydrase IX Targeted Polyaspartamide fluorescent Probes for Tumor imaging
Yu Zhang1,2, Fan Liu2,3, Chuntao Shao2
1College of Chemical and Material Engineering, Quzhou University, Quzhou, Zhejiang Province, 324000, People's Republic of China.
International Journal of Nanomedicine
|January 21, 2025
Summary
A novel fluorescent probe, sulfadiazine-polyaspartamide-naphthalimide (SD-PHEA-NI), shows promise for tumor detection. It effectively targets cancer cells, enabling clear fluorescence imaging for improved surgical guidance and patient outcomes.
Area of Science:
- Biomedical Engineering
- Chemical Biology
- Oncology
Background:
- Precise intraoperative tumor delineation is critical for surgical success, but current methods lack specificity and sensitivity.
- Fluorescence-guided surgery using tumor-targeting probes offers a promising alternative for distinguishing cancerous from healthy tissues.
- Contrast-enhanced fluorescent imaging aids in non-invasive cancer diagnosis.
Purpose of the Study:
- To synthesize and characterize a novel carbonic anhydrase IX-targeted fluorescent probe (SD-PHEA-NI).
- To evaluate the probe's potential for tumor-specific fluorescence imaging and its utility in image-guided surgery.
Main Methods:
- Synthesized SD-PHEA-NI by conjugating sulfadiazine (SD) and naphthalimide (NI) to a polyaspartamide carrier (PHEA).
- Characterized the probe using spectroscopy (FTIR, GPC, UV-Vis, NMR) and fluorescence assays.
- Assessed cellular uptake, cytotoxicity, and fluorescence imaging in cancer (B16F10, HeLa) and healthy (293T) cells.
Main Results:
- SD-PHEA-NI exhibited low cytotoxicity towards HeLa cells.
- B16F10 melanoma cells effectively uptook SD-PHEA-NI, producing strong green fluorescence signals.
- Healthy 293T cells showed only weak green fluorescence, indicating tumor specificity.
Conclusions:
- SD-PHEA-NI demonstrates potential as a fluorescent probe for tumor detection.
- This probe can enhance tumor diagnosis and image-guided surgical interventions.
- Real-time imaging and decision support may reduce recurrence rates and improve patient outcomes.
