Using the AP1 Transcription Factor FOSL1 to Assess the Exacerbation of Psoriasis

Area of Science:

• Dermatology
• Molecular Biology
• Biochemistry

Background:

• The transcription factor AP1 is vital for epidermal keratinocyte functions.
• AP1 subunits, like FOSL1, are implicated in skin cell proliferation, apoptosis, and differentiation.

Purpose of the Study:

• To investigate FOSL1 as a potential biomarker for psoriasis exacerbation.
• To evaluate FOSL1 protein and mRNA levels in patients undergoing bathwater PUVA therapy.
• To analyze FOSL1 expression in cultured keratinocytes and patient skin specimens.

Main Methods:

• Immunohistochemistry to determine FOSL1 distribution in skin.
• Quantitative PCR (qPCR) for gene expression analysis.
• Enzyme-linked immunosorbent assay (ELISA) for protein level assessment.

Main Results:

• FOSL1 was found to accumulate in lesional psoriatic skin.
• FOSL1 expression increased during disease flares and decreased after PUVA therapy.
• Silencing FOSL1 reduced the expression of ten disease-associated target genes.

Conclusions:

• FOSL1 demonstrates potential as a biomarker for psoriasis.
• FOSL1 expression correlates with psoriasis disease activity.
• FOSL1 is linked to the expression of genes involved in psoriasis pathogenesis.