Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Positive Regulator Molecules01:45

Positive Regulator Molecules

105.7K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
105.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

3.0K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.0K
Distribution of Cytoplasmic Content02:33

Distribution of Cytoplasmic Content

4.0K
Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of...
4.0K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries.

Cell & bioscience·2026
Same author

GTP cycling protein (GCP): An ancient player for allosteric gatekeeping.

mLife·2025
Same author

Filamentation of hCTPS1 with CTP.

Cell & bioscience·2025
Same author

Y12C mutation disrupts IMPDH cytoophidia and alters cancer metabolism.

The FEBS journal·2025
Same author

The Impact of Developmental and Metabolic Cues on Cytoophidium Formation.

International journal of molecular sciences·2024
Same author

Differential Cytoophidium Assembly between <i>Saccharomyces cerevisiae</i> and <i>Schizosaccharomyces pombe</i>.

International journal of molecular sciences·2024

Related Experiment Video

Updated: Jun 1, 2025

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
07:30

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Published on: December 7, 2019

9.1K

Cytoophidium complexes resonate with cell fates.

Yi-Lan Li1, Ji-Long Liu2,3

  • 1School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

Cellular and Molecular Life Sciences : CMLS
|January 21, 2025
PubMed
Summary

Metabolic enzymes form cellular structures called cytoophidia. Their assembly into complexes correlates with cell states, impacting cell fate and NAD biosynthesis.

Keywords:
Saccharomyces cerevisiaeAsn1Bna5CTPSCell fateCytoophidiumCytoophidium complexGlt1NADPrs5QuiescenceUra7

More Related Videos

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

9.9K
Microscopy of Fission Yeast Sexual Lifecycle
07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

14.5K

Related Experiment Videos

Last Updated: Jun 1, 2025

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
07:30

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Published on: December 7, 2019

9.1K
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

9.9K
Microscopy of Fission Yeast Sexual Lifecycle
07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

14.5K

Area of Science:

  • Cellular biology
  • Biochemistry
  • Metabolism

Background:

  • Metabolism is essential for life.
  • Cytoophidia, snake-like structures formed by metabolic enzymes like CTP synthase (CTPS), were discovered in 2010.
  • The distribution and interactions of different cytoophidia remain unclear.

Purpose of the Study:

  • To investigate the distribution and relationships of cytoophidia formed by five metabolic enzymes in Saccharomyces cerevisiae: Asn1, Bna5, CTPS (Ura7), Glt1, and Prs5.
  • To understand how cytoophidium assembly influences cell fate and metabolic pathways.

Main Methods:

  • Observation of cytoophidia formation and complex assembly in yeast cells.
  • Genetic manipulation to block cytoophidium assembly.
  • Analysis of cell phenotypes (quiescent vs. non-quiescent) and metabolic pathway activity (NAD biosynthesis).

Main Results:

  • Multiple cytoophidia can assemble into larger complexes.
  • Glt1 cytoophidia are prevalent in non-quiescent cells, while CTPS cytoophidia are more common in quiescent cells.
  • CTPS cytoophidia form complexes with Prs5 and Asn1.
  • Blocking CTPS cytoophidium assembly promotes a non-quiescent state, increases Glt1 and Bna5 cytoophidia, and inhibits NAD biosynthesis.
  • This non-quiescent phenotype can be rescued by interventions affecting Glt1 cytoophidia, nicotinic acid supplementation, or Sir2 overexpression.

Conclusions:

  • Cytoophidium complex composition is linked to distinct cell fates.
  • The assembly and interaction of cytoophidia play a crucial role in regulating cell quiescence and metabolic status, particularly NAD biosynthesis.