The PurR family transcriptional regulator promotes butenyl-spinosyn production in Saccharopolyspora pogona
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View abstract on PubMed
Summary
This summary is machine-generated.A high-production strain of Saccharopolyspora pogona was developed using Cobalt-60 γ-ray mutagenesis, leading to a significant increase in butenyl-spinosyn bioinsecticide production. The regulator TF00350 was identified as key to this enhanced synthesis.
Area Of Science
- Microbiology
- Biotechnology
- Genetics
Background
- Butenyl-spinosyn is a broad-spectrum bioinsecticide derived from Saccharopolyspora pogona.
- The regulatory mechanisms governing butenyl-spinosyn synthesis are not fully understood, limiting production optimization.
Purpose Of The Study
- To elucidate the regulatory mechanisms behind high butenyl-spinosyn production.
- To identify key genetic regulators for improving bioinsecticide yield.
Main Methods
- Cobalt-60 γ-ray mutagenesis was used to generate a high-production strain (S. pogona H2).
- Comparative transcriptomic analysis was performed between wild-type and high-production strains.
- Overexpression of the identified regulator TF00350 was analyzed.
Main Results
- A high-production strain (S. pogona H2) showed a 2.7-fold increase in butenyl-spinosyn yield.
- The transcriptional regulator TF00350 was identified and its overexpression increased production by 5.5-fold (141.5 mg/L).
- TF00350 regulates butenyl-spinosyn synthesis via modulation of cyclic di-GMP and cyclic di-AMP levels, impacting purine metabolism and biosynthesis genes.
Conclusions
- Cobalt-60 γ-ray mutagenesis is effective for generating improved bioinsecticide-producing strains.
- TF00350 acts as a positive regulator, significantly enhancing butenyl-spinosyn production through second messenger pathways.
- These findings offer valuable targets for further genetic mining and optimizing bioinsecticide production.
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