Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

282
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
282
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.6K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

1.4K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
1.4K
IR Spectrometers01:25

IR Spectrometers

1.1K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.1K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.3K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.3K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

2.9K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling Cross-Ring Dissociation Mechanisms of Hexoses in Collision-Induced Dissociation.

The journal of physical chemistry. A·2026
Same author

Mechanisms of Acquired Resistance Following Dual EGFR/MET Inhibition in MET-Amplified EGFR TKI-Resistant Lung Cancer.

Molecular cancer therapeutics·2026
Same author

Isomeric diversity of GlcNAc(Man<sub>3</sub>GlcNAc<sub>2</sub>) and GlcNAc<sub>2</sub>(Man<sub>3</sub>GlcNAc<sub>2</sub>) N-glycans in multicellular eukaryotes.

Scientific reports·2026
Same author

Adventitious carbon breaks symmetry in oxide contact electrification.

Nature·2026
Same author

Mutations in PilZ domains of newly discovered bacterial β-(1,3;1,4)-mixed linkage glucan synthases modulate polysaccharide production.

Carbohydrate polymers·2026
Same author

Mechanochemistry Activated by Confinement- and Shear-Induced Molecular Distortion.

Chemical reviews·2026
Same journal

Controlled Secondary Growth of CAU-1-NH<sub>2</sub> Membranes with Improved CO<sub>2</sub> Separation Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Facile Fabrication and Stable Mechanism of a Microscale Heavy Calcium Carbonate Suspension.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Polycationic Biocidal Coatings: The Mechanism of Their Interaction with Cells.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Atomic-Scale Displacement in Ordered SmMnO<sub>3</sub> Nanoislands.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Vacancy Defect Modulated Interfacial Thermal Transport and Phonon Localization in AlGaN/GaN Heterojunctions.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Immobilization of Ytterbium via Polyphenol Chemistry on Implant Materials for Enhanced Cytocompatibility and Antibacterial Properties.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jun 1, 2025

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion
06:48

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

Published on: May 9, 2025

179

Probing Surface Reactions on Multicomponent Glass Using Reflection-Absorption Infrared Spectroscopy.

Mason M Link1, Yen-Ting Lin1, Joy Banerjee2

  • 1Department of Chemical Engineering and Materials Research Institute, Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 21, 2025
PubMed
Summary
This summary is machine-generated.

Reflection-absorption infrared spectroscopy (RAIRS) identified molecular reactions on glass surfaces. Acetic acid vapor reacted with surface ions, releasing bound water, revealing surface chemistry insights.

More Related Videos

In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework
11:38

In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework

Published on: February 1, 2020

15.8K
The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight
10:27

The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight

Published on: October 11, 2016

9.5K

Related Experiment Videos

Last Updated: Jun 1, 2025

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion
06:48

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

Published on: May 9, 2025

179
In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework
11:38

In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework

Published on: February 1, 2020

15.8K
The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight
10:27

The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight

Published on: October 11, 2016

9.5K

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Spectroscopy

Background:

  • Elemental analysis of glass surfaces lacks molecular specificity.
  • Understanding glass surface reactivity is crucial for material applications.

Purpose of the Study:

  • To demonstrate reflection-absorption infrared spectroscopy (RAIRS) for detecting molecular species on glass surfaces.
  • To investigate the reaction of acetic acid vapor with alkali-free boroaluminosilicate and alkali aluminosilicate glasses.
  • To probe surface reaction sites with and without varied pH pretreatment.

Main Methods:

  • Utilized reflection-absorption infrared spectroscopy (RAIRS).
  • Employed acetic acid vapor as a model reactant.
  • Assisted analysis with theoretical calculations of spectral changes based on refractive indices.

Main Results:

  • Identified molecular species removed and produced at the glass surface.
  • Acetic acid reacted with surface modifier ions, forming monodentate acetate groups.
  • Observed desorption of 2-2.5 monolayers of strongly bonded water.

Conclusions:

  • RAIRS effectively detects molecular reactions on glass surfaces.
  • Surface reactivity with acetic acid was largely insensitive to pretreatment pH.
  • Pretreatment pH significantly impacted glass surface composition and network vibrations.