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Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

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Replicating Dynamic Immune Responses at Single-Cell Resolution within a Microfluidic Human Skin Equivalent.

Sarah A Hindle¹, Holly Bachas Brook^1,2, Alexandra Chrysanthou¹

¹Centre for Cell Biology and Cutaneous Research, Blizard Institute, Queen Mary University of London, 4 Newark Street, London, E1 2AT, UK.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|January 21, 2025

Summary

This summary is machine-generated.

Researchers developed a microfluidic human skin equivalent (HSE) to study skin immunity. This advanced model mimics inflammatory responses and immune cell behavior in human skin, aiding research into skin diseases and aging.

Keywords:

ageing inflammation microfluidic monocytes organ‐on‐chip skin

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Published on: May 17, 2021

Area of Science:

Immunology
Tissue Engineering
Microfluidics

Background:

Investigating human skin immunology in vitro is challenging.
Existing models lack the complexity to replicate dynamic immune cell interactions.

Purpose of the Study:

To develop a microfluidic human skin equivalent (HSE) for in vitro studies of skin immunology.
To model inflammatory responses and immune cell dynamics in human skin.

Main Methods:

Development of a microfluidic HSE with an embedded vascular microchannel.
Induction of keratinocyte inflammation to stimulate immune cell migration.
Single-cell transcriptomic analysis to study gene expression patterns.

Main Results:

The microfluidic HSE successfully supports immune cell delivery and migration.
Demonstrated dynamic, cell-specific gene expression during inflammatory responses.
Monocyte-derived cells differentiated into dermal macrophages within the HSE.
Accurately modeled age-associated immune dysfunction, including elevated monocyte recruitment.

Conclusions:

The microfluidic HSE is a viable tool for studying dynamic inflammatory immune responses in human skin.
This platform facilitates research into skin immunology mechanisms and age-related immune changes.