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Updated: Jun 1, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Enrichment and purification of trace substances from yew extracum by twin-column recycling chromatography with a step
Wei Xie1, Yuxue Wu2, Guangxia Jin2
1Ningbo Tech University, Ningbo 315100 China; Zhejiang University, Hangzhou 310058 China.
Abstract:
The concentration and purification of trace substances from natural products represent a bottleneck in current chromatographic separations. In this study, a twin-column recycling chromatography with a step solvent gradient method was proposed and successfully applied in the purification of unknown substances with tiny content in yew extracum. The method consists of two steps. Initially, the target substances were enriched from the yew extracum stock solution, followed by further separation and purification of the enriched substances in the second step. Ultimately, the target substances A and B were obtained with a purity of 99.7 % and 96.6 %, respectively. The proposed method has the potential to be applied in the preparation of trace substances for toxicological evaluation in natural product studies.
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