Related Experiment Video
Updated: Jun 1, 2025

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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
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Development of qPCR methods to detect and quantify the novel Fusarium graminearum 3ANX chemotype variant
Abbey Saunders1, Emily Johnstone1, Adam Foster1
1Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, PE C1A 4N6, Canada.
Journal of Microbiological Methods
|January 21, 2025
Abstract:
The devastating plant pathogen Fusarium graminearum produces mycotoxins including the novel 3ANX toxin. To detect 3ANX-producing isolates, SYBR Green and locked nucleic acid probe assays were developed, targeting 3ANX Tri1 polymorphisms. Assays were efficient with R2 > 0.99 and specific to 3ANX, detecting as few as 2 copies/μL.

