Related Experiment Video
Updated: Jun 1, 2025
07:55
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Published on: September 11, 2022
1.7K
The Shu complex interacts with the replicative helicase to prevent mutations and aberrant recombination
Adeola A Fagunloye1, Alessio De Magis2,3, Jordan H Little4
1University of Pennsylvania, School of Medicine, Department of Biochemistry and Biophysics, Philadelphia, PA, 19104, USA.
The EMBO Journal
|January 21, 2025
Summary
The yeast Shu complex prevents mutations during DNA replication by interacting with replication machinery. This interaction is crucial for DNA damage tolerance and avoiding errors.
Area of Science:
- Molecular Biology
- Genetics
- DNA Repair
Background:
- Homologous recombination (HR) is vital for DNA damage tolerance during replication.
- The yeast Shu complex is a conserved HR factor that prevents replication-associated mutagenesis.
Purpose of the Study:
- To investigate the role of the yeast Shu complex in coping with DNA damage during replication.
- To understand the interaction of the Shu complex with replication machinery.
Main Methods:
- Investigated yeast cell responses to MMS-induced DNA damage.
- Examined subunit interactions within the Shu complex and with replication factors (ORC, MCM).
- Performed evolutionary studies on Shu complex and replication-initiation factors.
Main Results:
- Csm2, a Shu complex subunit, binds to autonomous-replicating sequences (ARS).
- The Shu complex interacts with replication initiation complexes (ORC, MCM) via Psy3's N-terminus.
- These interactions are essential for DNA damage resistance and preventing mutations.
Conclusions:
- The Shu complex interacts with replication machinery to facilitate error-free bypass of DNA damage.
- Shu complex and replication initiation factors have co-evolved.
- This interaction is critical for maintaining genomic stability.
Related Concept Videos
02:31Homologous Recombination
50.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.1K
02:37Restarting Stalled Replication Forks
5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
01:02The DNA Replication Fork
35.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.5K
02:37DNA Damage can Stall the Cell Cycle
9.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.0K
02:15Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
02:38S-Cdk Initiates DNA Replication
4.6K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.6K