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Related Concept Videos

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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Related Experiment Video

Updated: May 31, 2025

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
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WDR74-Mediated Ribosome Biogenesis and Proteome Dynamics During Mouse Preimplantation Development.

Ayaka Kakihara1,2,3, Marino Maemura2,4, Atsushi Hatano3

  • 1Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|January 22, 2025
PubMed
Summary

Early mouse embryo development involves complex proteome changes. WDR74 deficiency impairs ribosome biogenesis and cell division, highlighting non-transcriptional regulation in embryogenesis.

Keywords:
pre‐implantation developmentpre‐implantation embryoproteomicsribosome biogenesis

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Area of Science:

  • Developmental Biology
  • Proteomics
  • Molecular Genetics

Background:

  • Preimplantation embryonic development relies on precise proteome and transcriptome regulation.
  • Maternal-to-zygotic transition is a key regulatory event.
  • Understanding protein dynamics is crucial for early embryogenesis.

Purpose of the Study:

  • To comprehensively analyze proteome dynamics during mouse preimplantation development.
  • To investigate the role of WD repeat-containing protein 74 (WDR74) in embryogenesis.
  • To explore non-transcriptional regulatory mechanisms in early development.

Main Methods:

  • Label-free quantitative proteomics was used to profile protein expression from oocytes to blastocysts.
  • CRISPR-Cas9 genome editing generated WDR74-deficient mouse embryos.
  • Proteomic data was integrated with transcriptomic data for comparative analysis.

Main Results:

  • 3490 proteins were identified, with 715 consistently detected across all stages.
  • Stage-specific proteomic changes were observed in translation, protein modification, and metabolism.
  • WDR74 deficiency severely impaired ribosome biogenesis, cell division, and morula stage progression, affecting key ribosomal proteins like RPL24 and RPL26.

Conclusions:

  • Proteomic analysis reveals dynamic protein changes crucial for preimplantation development.
  • WDR74 is essential for ribosome biogenesis and cell division, impacting embryogenesis.
  • Integrating proteomics and genome editing advances understanding of early developmental regulation.