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Updated: May 31, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Dual-Locked Enzyme-Activatable Fluorescence Probes for Precise Bioimaging.

Yang Liu1, Yuchen Yao1, Junhui Sha2

  • 1State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 211189, China.

ACS Biomaterials Science & Engineering
|January 22, 2025
PubMed
Summary

Dual-locked fluorescence probes offer enhanced precision for real-time enzyme imaging in disease diagnosis. These advanced probes overcome limitations of single-locked probes by detecting multiple biomarkers for improved accuracy.

Keywords:
Bioimagingcancer diagnosisdual stimulienzymefluorescence probe

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Area of Science:

  • Biomedical Imaging
  • Molecular Biology
  • Chemical Biology

Background:

  • Real-time enzyme visualization aids biological understanding and disease diagnosis.
  • Enzyme-activatable fluorescence probes enable sensitive and selective molecular imaging.
  • Single-locked probes can yield false positives and lack disease specificity.

Purpose of the Study:

  • To review recent advancements in dual-locked fluorescence probes for enzyme imaging.
  • To highlight breakthroughs in the last three years.
  • To discuss current challenges in the field.

Main Methods:

  • Summarizing recent progress in dual-locked fluorescence probe development.
  • Analyzing the application of dual enzymes or enzyme-stimulus pairs as activators.
  • Reviewing strategies for enhanced imaging precision and biomarker detection.

Main Results:

  • Dual-locked probes offer superior accuracy by requiring simultaneous activation by multiple targets.
  • These probes enable precise imaging of specific biological events and disease markers.
  • Recent innovations have significantly improved the sensitivity and selectivity of these probes.

Conclusions:

  • Dual-locked fluorescence probes represent a significant advancement over single-locked probes.
  • They provide enhanced precision for molecular imaging, crucial for accurate disease diagnosis and treatment guidance.
  • Further research is needed to address existing challenges and expand their clinical applications.