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Published on: August 8, 2016
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Integrated omics analyses elucidate acetaminophen biodegradation by Enterobacter sp. APAP_BS8.
Bhavana Pandey1, Anand Kumar Pandey2, Suresh Kumar Dubey1
1Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
Journal of Environmental Management
|January 22, 2025
Summary
Enterobacter sp. APAP_BS8 effectively degrades acetaminophen (APAP), a common environmental contaminant. This study elucidates the genomic and proteomic mechanisms behind its potent bioremediation capabilities.
Area of Science:
- Environmental microbiology
- Bioremediation
- Pharmaceutical pollution
Background:
- Acetaminophen (APAP) is a widely used pharmaceutical and a significant environmental contaminant.
- Inefficient wastewater treatment leads to the widespread presence of APAP, necessitating effective remediation strategies.
- Microbial degradation offers a sustainable approach to removing APAP from contaminated environments.
Purpose of the Study:
- To evaluate the efficacy of Enterobacter sp. APAP_BS8 for acetaminophen degradation.
- To elucidate the underlying genomic, proteomic, and metabolomic mechanisms of APAP biodegradation.
- To explore the potential of Enterobacter sp. APAP_BS8 in developing sustainable bioremediation technologies.
Main Methods:
- Microcosm experiments to assess APAP degradation efficiency by Enterobacter sp. APAP_BS8.
- Whole genome sequencing to identify potential APAP-degrading genes.
- Proteomic analysis to confirm the expression of relevant proteins.
- Metabolomic analysis to identify degradation intermediates.
- Molecular docking and simulations to understand enzyme-substrate interactions.
Main Results:
- Enterobacter sp. APAP_BS8 degraded approximately 88% of APAP (300 mg kg⁻¹) in 16 days.
- Genomic analysis identified key genes including deaminated glutathione amidase and oxidoreductases involved in degradation.
- Proteomic data confirmed increased expression of proteins associated with these identified genes.
- Metabolomic analysis revealed hydroxyquinol, 4-aminophenol, and 3-hydroxy-cis, cis-muconate as degradation intermediates.
- Molecular docking supported the binding of intermediates to the catalytic sites of identified enzymes.
Conclusions:
- Enterobacter sp. APAP_BS8 possesses a robust enzymatic system for acetaminophen degradation.
- The study provides a mechanistic understanding of APAP biodegradation by this bacterial strain.
- These findings support the development of Enterobacter sp. APAP_BS8 for practical bioremediation applications.
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