Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.1K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

867
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
867
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.2K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

4.7K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[ATM/H2AX and repair of sperm-DNA damage during cryopreservation].

Zhonghua nan ke xue = National journal of andrology·2011
Same author

Predicting accident frequency at their severity levels and its application in site ranking using a two-stage mixed multivariate model.

Accident; analysis and prevention·2011
Same author

Photothermally enhanced photodynamic therapy delivered by nano-graphene oxide.

ACS nano·2011
Same author

[Characteristics of soil respiration in Phyllostachys edulis forest in Wanmulin Natural Reserve and related affecting factors].

Ying yong sheng tai xue bao = The journal of applied ecology·2011
Same author

Quality changes in sea urchin (Strongylocentrotus nudus) during storage in artificial seawater saturated with oxygen, nitrogen and air.

Journal of the science of food and agriculture·2011
Same author

Global effect of an RNA polymerase β-subunit mutation on gene expression in the radiation-resistant bacterium Deinococcus radiodurans.

Science China. Life sciences·2011

Related Experiment Video

Updated: May 31, 2025

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
06:58

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

569

Ribosomal protein L6 suppresses hepatocellular carcinoma by modulating FBXO22-mediated p53 degradation.

Zhen Lei1, Yiming Luo2, Qinggang Fu2

  • 1Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Clinical Medicine Research Center for Hepatic Surgery of Hubei Province, Key Laboratory of Organ Transplantation, Ministry of Education, NHC Key Laboratory of Organ Transplantation, Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, Hubei 430030, PR China; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang 832008, PR China.

Cellular Signalling
|January 22, 2025
PubMed
Summary
This summary is machine-generated.

Ribosomal protein L6 (RPL6) is decreased in liver cancer (HCC), hindering tumor growth. RPL6 stabilizes p53, inhibiting HCC cell proliferation and suggesting its potential as a therapeutic target.

Keywords:
HCCRPL6Ubiquitinationp53

More Related Videos

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.0K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.1K

Related Experiment Videos

Last Updated: May 31, 2025

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
06:58

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

569
SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.0K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.1K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ribosomal protein L6 (RPL6) plays a role in various cancers, but its function in hepatocellular carcinoma (HCC) is not well understood.
  • Understanding the molecular mechanisms of HCC progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of RPL6 in hepatocellular carcinoma (HCC) progression.
  • To elucidate the molecular mechanism by which RPL6 affects HCC growth.

Main Methods:

  • Analysis of RPL6 expression levels in HCC tissues and correlation with clinical parameters.
  • In vitro functional assays to assess the impact of RPL6 on HCC cell proliferation.
  • Xenograft tumor models to evaluate RPL6's effect on tumor growth.
  • Investigation of the interaction between RPL6, FBXO22, and p53 using molecular biology techniques.

Main Results:

  • RPL6 expression is significantly downregulated in HCC tissues.
  • Decreased RPL6 levels correlate with larger tumor size, vascular invasion, and poorer prognosis.
  • RPL6 overexpression inhibits HCC cell proliferation and xenograft tumor growth.
  • RPL6 binds to FBXO22, preventing p53 ubiquitination and degradation, thereby stabilizing p53 and inhibiting cell growth.

Conclusions:

  • RPL6 acts as a tumor suppressor in HCC by inhibiting cell proliferation through the FBXO22/p53 signaling pathway.
  • RPL6's role in stabilizing p53 highlights its importance in HCC pathogenesis.
  • RPL6 represents a potential biomarker and therapeutic target for hepatocellular carcinoma.