Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

498
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
498
  1. Home
  3. Pine (pinus Koraiensis) Nut Oil Ameliorates Cholesterol Homeostasis And Inflammation Via Modulating The Mir-34a/122 Pathways In The Liver Of Rats Fed A High-cholesterol Diet
  1. Home
  3. Pine (pinus Koraiensis) Nut Oil Ameliorates Cholesterol Homeostasis And Inflammation Via Modulating The Mir-34a/122 Pathways In The Liver Of Rats Fed A High-cholesterol Diet

Related Experiment Video

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.2K

Pine (Pinus koraiensis) Nut Oil Ameliorates Cholesterol Homeostasis and Inflammation via Modulating the miR-34a/122

Yunji Lee1, Mak-Soon Lee2, Jumi Lee1

  • 1Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Republic of Korea; Graduate Program in System Health Science and Engineering, Ewha Womans University, Seoul, Republic of Korea.

The Journal of Nutrition
|January 22, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
cholesterol homeostasisinflammationlivermicroRNApine nut oil

More Related Videos

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
11:03

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Published on: September 9, 2021

3.8K
Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss
07:03

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Published on: July 19, 2024

765

Related Experiment Videos

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.2K
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
11:03

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Published on: September 9, 2021

3.8K
Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss
07:03

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Published on: July 19, 2024

765

Pine nut oil (PNO) supplementation improved cholesterol levels and reduced inflammation in rats fed a high-cholesterol diet. These benefits are linked to the regulation of miR-34a/122 pathways in the liver.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nutritional Science

Background:

  • Pine (Pinus koraiensis) nut oil (PNO) shows potential benefits for hepatic triglyceride accumulation and atherosclerosis.
  • MicroRNAs (miRs) play a role in various diseases by modulating physiological processes.
  • The precise mechanism of PNO's effect on miRs in hepatic cholesterol homeostasis and inflammation is not fully understood.

Purpose of the Study:

  • To investigate the impact of PNO on miR-34a/122 pathways.
  • To examine PNO's effects on cholesterol homeostasis and liver inflammation.
  • To utilize a high-cholesterol diet (HCD) rat model to study these effects.

Main Methods:

  • Rats were fed a cholesterol-free diet, an HCD, or an HCD with 5% PNO for 4 weeks.
  • Evaluated serum and liver lipid profiles, performed histological analysis.
  • Analyzed gene and protein expression, including liver X receptor α, cholesterol efflux transporters, inflammatory cytokines, and miR-34a/122.

    • Main Results:

    • PNO improved hepatic lipid profiles and increased HDL cholesterol in HCD rats.
    • PNO upregulated cholesterol efflux genes (LXRα, ABCA1/G1) and downregulated inflammatory cytokines (TNF-α, IL-6, IL-1β).
    • PNO decreased NF-κB activity, nitric oxide production, and hepatic miR-34a and miR-122 expression.

    Conclusions:

    • PNO supplementation positively influences cholesterol homeostasis and inflammation in the liver.
    • These effects are, in part, mediated by the regulation of miR-34a/122 pathways.
    • PNO demonstrates potential as a therapeutic agent for managing liver health under high-cholesterol conditions.