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Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Updated: May 31, 2025

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

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Published on: December 22, 2014

Diverse Cre recombinase expression pattern in Albumin-Cre driver rats.

Saeko Ishida¹, Keiko Taguchi^2,3, Ryuya Iida¹

¹Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

Experimental Animals

|January 22, 2025

Summary

Optimizing Cre-driver rat models is crucial for genetic research. This study found insertion orientation and promoter origin significantly impact Cre expression, guiding the development of more precise liver-specific models.

Keywords:

Cre-loxP genome editing histology liver rats

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Area of Science:

Genetics and Genomics
Animal Models
Molecular Biology

Background:

Rats are vital biomedical research models due to their physiology, complementing mouse studies.
Cre-driver rat models offer spatiotemporal gene expression control but require further development.
Genome editing advances enable genetically modified rat strains for experimental studies.

Purpose of the Study:

To develop and characterize liver-targeting Cre knock-in and reporter knock-in rats.
To evaluate Cre recombinase expression profiles under various genetic contexts.
To provide insights for optimizing Cre-driver rat models for tissue specificity.

Main Methods:

Generation of liver-targeting Cre knock-in rats.
Generation of reporter knock-in rats.
Evaluation of Cre recombinase expression patterns based on insertion orientation and promoter origin.

Main Results:

Insertion orientation and promoter origin critically influence Cre expression patterns.
Forward insertion of Albumin promoter-driven Cre at ROSA26 locus led to ubiquitous expression.
Reverse insertion predominantly confined Cre expression to the liver.
Endogenous Albumin promoter-driven Cre showed unexpected expression in non-liver tissues.

Conclusions:

Rigorous characterization is essential for Cre-based transgenic systems.
Promoter origin, insertion site, and orientation are key factors in Cre-driver rat model design.
Findings enhance strategies for tissue-specific gene expression in functional genomics and disease modeling.