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Updated: Jun 14, 2025

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
05:27

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

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Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a

Zhouzhou Tang1,2, Zhihao Li2, Guofeng Wu3

  • 1Department of Spinal Surgery, Zhujiang Hospital, Southern Medical University, 253 Industrial Avenue Central Guangzhou, Guangdong510280, Guangzhou, Guangdong510280, China.

Scientific Reports
|January 22, 2025
PubMed

Insights

The long noncoding RNA DHRS4 antisense RNA 1 (DHRS4-AS1) is downregulated in osteosarcoma (OS). Restoring DHRS4-AS1 inhibits OS cell proliferation and promotes apoptosis, suggesting its therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is the most common primary malignant bone tumor.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • The specific function of DHRS4 antisense RNA 1 (DHRS4-AS1) in OS is largely unknown.

Purpose of the Study:

  • To investigate the expression, function, and molecular mechanism of DHRS4-AS1 in osteosarcoma.
  • To determine if DHRS4-AS1 can serve as a potential therapeutic target for OS.

Main Methods:

  • Quantitative PCR to detect DHRS4-AS1 expression in OS cell lines.
  • Gain- and loss-of-function experiments to assess the impact of DHRS4-AS1 on OS cell proliferation and apoptosis.
  • Bioinformatics analysis and rescue experiments to elucidate the underlying molecular mechanism.

Main Results:

  • DHRS4-AS1 expression was found to be downregulated in OS cell lines.
  • DHRS4-AS1 depletion promoted OS cell proliferation and inhibited apoptosis.
  • DHRS4-AS1 overexpression exhibited opposite effects, suppressing proliferation and enhancing apoptosis.
  • DHRS4-AS1 was shown to inhibit OS progression by regulating the microRNA-362-5p/aminopeptidase puromycin sensitive axis.

Conclusions:

  • DHRS4-AS1 plays a crucial role in regulating osteosarcoma cell proliferation and apoptosis.
  • DHRS4-AS1 functions as a tumor suppressor in osteosarcoma.
  • DHRS4-AS1 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
DHRS4-AS1Long noncoding RNAsMiR-362-5pNPEPPSOsteosarcoma

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