Complete genome sequence of Pseudomonas aeruginosa YK01, a sequence type 16 isolated from a patient with keratitis
Shuo Jiang1, Mengmin Ye2, Ke Liu3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Objectives:
Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, is frequently associated with multidrug resistance and global epidemic outbreaks, contributing significantly to morbidity and mortality in hospitalized patients. However, P. aeruginosa belonging to the sequence type (ST) 16 was rarely reported. Here, this report presents the complete genome sequence of YK01, a ST16 P. aeruginosa isolate from a patient with keratitis. The complete reference genome of P. aeruginosa YK01 is expected to provide valuable data for investigating its genomic population, enhancing understanding of genetic basis of P. aeruginosa species complex.
Data Description:
A complete genome of 6.3 Mb was obtained for P. aeruginosa YK01 by combining Illumina 150-bp short reads and Nanopore long reads. The assembly is fully complete with chromosomal genome size of 6,183,266 bp, presenting a GC content of 66.7%, and a plasmid with the size of 46,067 bp, presenting GC content of 59.0%. Predicted chromosomal genomic features include 5,709 CDS, 12 rRNAs, 63 tRNAs, 4 ncRNAs, and 5,788 genes. To our knowledge, this genome data represents the first complete genome of P. aeruginosa ST16, providing crucial information for further comparative genome analysis.
Insights
This study presents the first complete genome sequence of Pseudomonas aeruginosa sequence type 16 (ST16), a rare but significant pathogen. This genomic data aids in understanding the genetic basis of this multidrug-resistant bacterium.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen linked to multidrug resistance and hospital-acquired infections.
- Sequence type 16 (ST16) Pseudomonas aeruginosa is rarely reported, despite the pathogen's significant contribution to morbidity and mortality.
Purpose of the Study:
- To present the complete genome sequence of a ST16 Pseudomonas aeruginosa isolate (YK01) from a keratitis patient.
- To provide a valuable genomic resource for studying the P. aeruginosa species complex and its population structure.
Main Methods:
- A hybrid assembly approach combining Illumina short reads and Nanopore long reads was used.
- The complete genome was assembled to 6.3 Mb, including a chromosome of 6,183,266 bp and a plasmid of 46,067 bp.
Main Results:
- The P. aeruginosa YK01 genome comprises 5,709 coding sequences (CDS), 12 rRNAs, 63 tRNAs, and 4 non-coding RNAs.
- This represents the first reported complete genome for P. aeruginosa ST16, offering crucial data for comparative analysis.
Conclusions:
- The availability of the complete P. aeruginosa ST16 genome sequence is essential for future research.
- This genomic data will enhance understanding of the genetic underpinnings of P. aeruginosa populations and virulence.
