Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic Divergence Shaped the Genetic Regulation of Meiotic Homologous Recombination in Brassica Allopolyploids.

Molecular biology and evolution·2025
Same author

Alternating between even and odd ploidy levels switches on and off the recombination control, even near the centromeres.

The Plant cell·2024
Same author

MiniRead: A simple and inexpensive do-it-yourself device for multiple analyses of micro-organism growth kinetics.

Yeast (Chichester, England)·2024
Same author

Quantitative modelling of fine-scale variations in the <i>Arabidopsis thaliana</i> crossover landscape.

Quantitative plant biology·2023
Same author

SeSAM: software for automatic construction of order-robust linkage maps.

BMC bioinformatics·2022
Same author

Evidence of an additional centre of apple domestication in Iran, with contributions from the Caucasian crab apple Malus orientalis Uglitzk. to the cultivated apple gene pool.

Molecular ecology·2022

Related Experiment Video

Updated: May 31, 2025

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.3K

CAYSS: Package for Automatic Cytometry Analysis of Yeast Spore Segregation.

Xavier Raffoux1, Matthieu Falque1

  • 1INRAE, CNRS, AgroParisTech, Université Paris-Saclay, Gif-sur-Yvette, France.

Yeast (Chichester, England)
|January 23, 2025
PubMed
Summary

CAYSS software automates yeast recombination analysis from flow cytometry data. This tool saves significant time and ensures reproducible results for measuring genetic diversity and adaptation.

Keywords:
FACSbudding yeastcrossover interferenceflow cytometrymeiotic recombination rate

More Related Videos

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis
09:28

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis

Published on: May 25, 2018

19.2K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

998

Related Experiment Videos

Last Updated: May 31, 2025

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.3K
Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis
09:28

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis

Published on: May 25, 2018

19.2K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

998

Area of Science:

  • Genetics
  • Evolutionary Biology
  • Computational Biology

Background:

  • Meiotic recombination generates genetic diversity crucial for adaptation.
  • Quantifying recombination rates is challenging due to large offspring sample sizes.
  • Flow cytometry of yeast spores offers an efficient method for recombination measurement.

Purpose of the Study:

  • To introduce CAYSS, a software package for automated analysis of yeast meiotic recombination.
  • To validate CAYSS by comparing its results to manual analysis of flow cytometry data.

Main Methods:

  • Development of the CAYSS package for automated analysis of yeast spore fluorescence data.
  • Application of Maximum-Likelihood estimation for fluorescence extinction within CAYSS.
  • Comparative analysis of recombination rates and interference between manual and CAYSS methods.

Main Results:

  • CAYSS automatically reproduces manual analysis steps for yeast recombination.
  • Recombination rates and interference measurements showed less than 3% average difference between manual and CAYSS analyses.
  • Strong correlations (R² > 0.9) were observed between the two analysis methods.

Conclusions:

  • CAYSS significantly reduces human time required for yeast recombination analysis.
  • The CAYSS package provides highly reproducible and accurate results compared to manual methods.
  • Automated analysis with CAYSS enhances the efficiency of studying genetic diversity and adaptation.