Effects of cold storage on double integrating sphere optical property measurements of porcine dermis and subcutaneous fat from 400 to 1100 nm
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View abstract on PubMed
Summary
This summary is machine-generated.Skin optical properties are influenced by storage. Refrigeration and freezing cause minor changes in absorption and scattering coefficients, aiding future research planning.
Area Of Science
- Biomedical Optics
- Optical Imaging
- Tissue Optics
Background
- Accurate skin optical properties are crucial for computational modeling and optical imaging system optimization.
- Published optical property values exhibit significant variability due to sample handling and experimental differences.
Purpose Of The Study
- To investigate the impact of various storage conditions (refrigeration, freezing, flash freezing) on excised skin optical properties.
- To analyze spectral changes in absorption and scattering coefficients across the 400-1100 nm range.
Main Methods
- Utilized a double integrating sphere system for optical measurements.
- Employed the inverse adding-doubling method to determine absorption (<math> </math>) and reduced scattering (<math> </math>) coefficients.
- Examined porcine dermis and subcutaneous fat samples before and after storage.
Main Results
- Small average changes in absorption coefficients (<math> </math>) were observed for both dermis and subcutaneous tissue post-storage.
- Reduced scattering coefficients (<math> </math>) showed negligible average changes across storage conditions.
- The most significant variations were noted in the hemoglobin absorption region.
Conclusions
- Findings provide context for the variability in published skin optical properties.
- Informed selection of sample storage conditions can improve the reliability of future optical measurements.
- Results aid in optimizing study design and maximizing the utility of limited tissue samples.
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