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Updated: May 31, 2025

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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Senolytic Targeting of Anti-Apoptotic Bcl Family Increases Cell Death in UV-Irradiated Senescent Melanocytes: Search

Jin Cheol Kim1,2, Na Yeon Kim1,3, Yeongeun Kim1,2

  • 1Department of Dermatology, Ajou University School of Medicine; Suwon, Suwon, Korea.

Experimental Dermatology
|January 23, 2025
PubMed

Insights

Senescent melanocytes contribute to skin aging. ABT-737 and ABT-263 effectively cleared these cells via apoptosis, indicating potential for anti-aging skin treatments.

Area of Science:

  • Dermatology
  • Cell Biology
  • Gerontology

Background:

  • Senescent melanocytes are implicated in age-related skin changes and hyperpigmentation.
  • Cellular senescence contributes to the aging phenotype of the skin.

Purpose of the Study:

  • To evaluate the senolytic potential of various compounds on UV-induced senescent melanocytes.
  • To investigate the mechanisms underlying the senolytic activity of effective compounds.

Main Methods:

  • UV irradiation was used to induce senescence in melanocytes.
  • Senescent cells were treated with known senolytic chemicals and natural compounds.
  • Key markers of senescence (SA-β-Gal, p16INK4A, p21Waf1) and apoptosis (cleaved caspase-3) were assessed.

Main Results:

  • ABT-737 and ABT-263 significantly reduced senescent melanocyte populations.
  • These drugs decreased the expression of senescence markers p16INK4A and p21Waf1.
  • Senolytic effects were linked to increased cleaved caspase-3, blocked by the caspase inhibitor Z-VAD.

Conclusions:

  • ABT-737 and ABT-263 demonstrate senolytic capacity by inducing apoptosis in senescent melanocytes.
  • The findings suggest these compounds may be beneficial for targeting age-related skin conditions.
Keywords:
ABT‐263ABT‐737senescent melanocytesenolyticskin ageing

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