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MA104 cell line is permissive for human bocavirus 1 infection
Jielin Tang1,2, Sijie Chen3, Yijun Deng1,2
1Guangzhou National Laboratory, Guangzhou, China.
Journal of Virology
|January 23, 2025
Summary
Researchers identified MA104 cells as permissive to human bocavirus 1 (HBoV1) replication. This breakthrough enables studying HBoV1 life cycle and developing antiviral therapies for infections.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- Human bocavirus 1 (HBoV1) is an emerging pathogen causing significant respiratory illnesses.
- Current research on HBoV1 is limited by the absence of suitable cell culture models for its replication.
Purpose of the Study:
- To identify a permissive cell line supporting the complete life cycle of HBoV1.
- To establish a cell-based model for investigating HBoV1 pathogenesis and developing antiviral strategies.
Main Methods:
- Screening of 29 human and 7 animal cell lines for HBoV1 susceptibility.
- Characterization of the HBoV1 life cycle in permissive cells.
- RNA-sequencing to analyze host responses to HBoV1 infection.
Main Results:
- MA104 cells were identified as a permissive cell line supporting the complete HBoV1 life cycle.
- Viral entry, replication, and infectious progeny production were confirmed in MA104 cells.
- Suppression of the interferon pathway enhanced viral replication; host pathways like PI3K-Akt were activated.
Conclusions:
- MA104 cells provide the first viable model for culturing and studying HBoV1.
- This model is crucial for advancing HBoV1 virology, pathogenesis research, and antiviral/vaccine development.
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