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Unlocking Probiotic Potential: Physicochemical Approaches to Evaluate Probiotic Bacterial Adhesion Potential to the
Thị-Thanh-Trúc Phùng1, Sébastien Dupont1, Laurent Beney1
1L'institut Agro, Université Bourgogne Europe, INRAe, UMR PAM, Dijon, F-21000, France.
Molecular Nutrition & Food Research
|January 23, 2025
Summary
This study reveals how bacterial adhesion in the gut impacts probiotic effectiveness. Escherichia coli and Bifidobacterium infantis show the highest gut adhesion potential, using novel physicochemical methods.
Area of Science:
- Microbiology
- Biophysics
- Gastroenterology
Background:
- Bacterial adhesion in the gut is crucial for probiotic efficacy.
- Assessing bacterial adhesion in the complex gut environment presents challenges.
- Existing methods do not fully capture adhesion to intestinal mucus.
Purpose of the Study:
- To explore bacterial adhesion mechanisms and potential in the gut.
- To evaluate five bacterial strains: Escherichia coli, Lactiplantibacillus plantarum, Faecalibacterium duncaniae, Bifidobacterium longum, and Bifidobacterium longum subsp. infantis.
- To introduce novel physicochemical approaches for assessing gut bacterial adhesion.
Main Methods:
- Evaluation of bacterial morphology, rheology, and surface properties.
- Development of a new method for bacterial adhesion to mucus using contact angle, surface tension, and work of adhesion.
- Multicriteria evaluation of bacterial adhesion from initial transport to irreversible adhesion.
Main Results:
- Intestinal mucus is hydrophilic, favoring adhesion of hydrophilic bacteria like E. coli and B. infantis.
- E. coli and B. infantis exhibited the highest overall gut adhesion potential.
- Adhesion potential ranking: E. coli, B. infantis, L. plantarum, B. longum, F. duncaniae.
Conclusions:
- Novel physicochemical methods provide comprehensive insights into bacterial gut adhesion.
- Hydrophilicity plays a key role in bacterial adhesion to intestinal mucus.
- E. coli and B. infantis are identified as having superior gut adhesion properties among the tested strains.
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