Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure
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Updated: May 31, 2025
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
Published on: July 12, 2022
Ranhao Zhang1, Yuan Shen2, Xueming Li3
1Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China; State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; Department of Electronic Engineering, Tsinghua University, Beijing 100084, China; Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China.
Abstract:
Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). Here, we present a protocol for CTF estimation of the cryoET tilt series with CTFMeasure. CTFMeasure can estimate the CTF parameters together with the absolute tilt angle offset of the sample. We describe steps for configuring the input files and estimating the CTF parameters and the absolute tilt angle offset of the input tilt series. We then detail procedures for visualizing the power spectra and analyzing the output files. For complete details on the use and execution of this protocol, please refer to Zhang et al.1.
