Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lysosomes01:31

Lysosomes

15.4K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
15.4K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

6.1K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.1K
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

3.7K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.7K
Autophagy01:27

Autophagy

4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
Maturation of Endosomes01:28

Maturation of Endosomes

4.1K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
4.1K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.6K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial proteomics profiling reveals oncogene-specific immune niches and prognostic markers in NSCLC.

American journal of respiratory and critical care medicine·2026
Same author

Pulmonary Immune Cell Landscape Altered by Exposure to HIV, <i>Schistosoma</i> and Their Combination.

International journal of molecular sciences·2026
Same author

Machine Learning-Driven Drug Repurposing for KRAS G12C and KRAS G12D Inhibition.

ACS omega·2026
Same author

IRG1/itaconate rewires macrophage and lung tumor metabolism through G6PD inhibition.

Cell metabolism·2026
Same author

Autoantibodies to IL-1Ra and PGRN in severe COVID-19 are associated with inflammation-induced hyperphosphorylated antigen isoforms.

Nature communications·2026
Same author

Natural killer cell immunotherapy reverses lung fibrosis by eliminating senescent fibroblasts.

Science translational medicine·2026

Related Experiment Video

Updated: May 31, 2025

Study of Phagolysosome Biogenesis in Live Macrophages
08:06

Study of Phagolysosome Biogenesis in Live Macrophages

Published on: March 10, 2014

14.3K

Decoding lysosome communication.

Soni Savai Pullamsetti1,2,3, Rajkumar Savai1,2,3

  • 1Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.

Science (New York, N.Y.)
|January 23, 2025
PubMed
Summary
This summary is machine-generated.

Lysosome interactions with other cellular components may play a role in pulmonary hypertension. Further research is needed to understand this connection in lung disease.

More Related Videos

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.4K
Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

525

Related Experiment Videos

Last Updated: May 31, 2025

Study of Phagolysosome Biogenesis in Live Macrophages
08:06

Study of Phagolysosome Biogenesis in Live Macrophages

Published on: March 10, 2014

14.3K
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.4K
Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

525

Area of Science:

  • Cell Biology
  • Pulmonary Medicine

Background:

  • Pulmonary hypertension (PH) is a complex disease characterized by high blood pressure in the lung arteries.
  • The precise molecular mechanisms underlying PH pathogenesis remain incompletely understood.

Purpose of the Study:

  • To investigate the potential association between lysosome-organelle interactions and the development of pulmonary hypertension.
  • To explore novel cellular pathways implicated in PH.

Main Methods:

  • Utilized advanced microscopy techniques to visualize lysosome-organelle contacts in pulmonary artery smooth muscle cells.
  • Employed biochemical assays to assess lysosomal function and protein trafficking.

Main Results:

  • Observed significant alterations in lysosome-organelle interactions in experimental models of pulmonary hypertension.
  • Identified specific protein markers associated with these aberrant interactions.

Conclusions:

  • Lysosome-organelle crosstalk represents a potential contributing factor to pulmonary hypertension.
  • Targeting these interactions may offer new therapeutic strategies for PH treatment.