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Shining a Light on Peptide and Protein Synthesis: Light-Emitting-Diode-Driven Desulfurization of Cysteine to Alanine
Mateusz Waliczek1, Piotr Stefanowicz1
1Faculty of Chemistry, University of Wrocław, Joliot-Curie 14 Street, 50-383 Wrocław, Poland.
Abstract:
Studies presenting visible-light-induced desulfurization of peptides containing a cysteine residue have been carried out. This transformation driven by light-emitting-diode-type light proceeds with high efficiency in an aqueous solution at room temperature and involves the use of a catalytic amount of photosensitizer, Rose Bengal. The procedure has been tested on model synthetic peptides, lysozyme C and α-crystallin, and successfully applied to a one-pot native chemical ligation (NCL)-desulfurization protocol.
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