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Diagnostic value of miR-193a-5p in severe pneumonia and its correlation with prognosis
Xiaoji Su1, Lijuan Zhu1, Jiajia Zhuo1
1Clinical Laboratory, Anhui Hospital, Children's Hospital of Fudan University, No. 39, Wangjiang East Road, Hefei, Anhui, 230011, China.
Insights
Elevated serum miR-193a-5p levels in children with severe pneumonia may aid early diagnosis and prognosis. This microRNA shows potential as a biomarker for identifying disease severity and predicting patient outcomes.
Area of Science:
- Pediatric Medicine
- Molecular Biology
- Biomarker Discovery
Background:
- Severe pneumonia poses a significant threat to children's lives due to rapid progression and complications like respiratory failure.
- Identifying reliable biomarkers is crucial for timely diagnosis and effective management of pediatric severe pneumonia.
Purpose of the Study:
- To investigate the clinical significance of miR-193a-5p in children diagnosed with severe pneumonia.
- To evaluate miR-193a-5p as a potential diagnostic and prognostic biomarker for severe pneumonia in pediatric patients.
Main Methods:
- Serum miR-193a-5p levels were quantified using RT-qPCR in 150 children with severe pneumonia and 150 healthy controls.
- Correlations with inflammatory markers (CRP, WBC, neutrophil count, NLR) were assessed. Diagnostic and prognostic values were analyzed via ROC curves, Kaplan-Meier analysis, and logistic regression.
Main Results:
- Serum miR-193a-5p levels were significantly higher in children with severe pneumonia, correlating positively with CRP, WBC, neutrophil count, and NLR.
- miR-193a-5p demonstrated good diagnostic accuracy (AUC=0.862) for severe pneumonia.
- Elevated miR-193a-5p expression was associated with poor prognosis and predicted adverse outcomes in pediatric patients.
Conclusions:
- Serum miR-193a-5p is markedly elevated in children with severe pneumonia.
- miR-193a-5p holds potential as a valuable biomarker for early diagnosis and prognostic assessment in pediatric severe pneumonia.
Background:
Severe pneumonia is a common disease in children, with rapid progression and easy complications of respiratory failure, endangering the lives of children. This study aimed to elucidate the clinical significance of miR-193a-5p in severe pneumonia and to provide a new biomarker for the disease.
Methods:
A total of 150 children with severe pneumonia and an equal number of healthy children were selected for analysis. Serum miR-193a-5p levels were detected by RT-qPCR. The correlation of miR-193a-5p with CRP, WBC, neutrophil count, and NLR was assessed by Spearman analysis. The diagnostic and prognostic value of miR-193a-5p in severe pneumonia was analyzed using ROC curves. The relationship between miR-193a-5p and the prognosis of severe pneumonia was evaluated using the Kaplan-Meier curve and a multivariate logistic analysis.
Results:
Serum levels of miR-193a-5p were markedly elevated in children with severe pneumonia and exhibited a positive correlation with CRP, WBC, neutrophil count, and NLR. miR-193a-5p could effectively distinguish children with severe pneumonia from healthy children, with an AUC, sensitivity, and specificity of 0.862, 70.67%, and 88.67%, respectively. Serum miR-193a-5p expression was increased in children with poor prognosis and had a predictive value for patient prognosis. High expression of miR-193a-5p was linked to survival in children with severe pneumonia and was a risk factor for adverse prognosis.
Conclusion:
Serum levels of miR-193a-5p were markedly elevated in children with severe pneumonia, which may be of significance for the early diagnosis of the disease and prognostic assessment.
