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Updated: May 31, 2025

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Mechanically Triggered Protein Desulfurization.

Dongyang Han1, Yan Cui1, Xiangyu Deng1

  • 1New Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.

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|January 24, 2025
PubMed
Summary
This summary is machine-generated.

Ultrasonic energy offers a new method for protein desulfurization. This ultrasound-induced desulfurization (USID) strategy is simple, broadly applicable, and compatible with sensitive functional groups in chemical protein synthesis.

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Area of Science:

  • Chemical Biology
  • Biochemistry
  • Organic Chemistry

Background:

  • Native chemical ligation and postligation desulfurization are key techniques in modern chemical protein synthesis.
  • Existing desulfurization methods can be harsh and lack functional group compatibility.

Purpose of the Study:

  • To develop a novel, robust, and widely applicable protein desulfurization strategy.
  • To introduce an ultrasound-induced desulfurization (USID) method for chemical protein synthesis.

Main Methods:

  • Utilized ultrasonic energy in a simple cleaning bath with titanium dioxide as a sonosensitizer.
  • Applied the USID strategy to various peptides and proteins, including those with sensitive functional groups like thiazolidine and serotonin.
  • Investigated the mechanism of USID using low-frequency and low-intensity ultrasonication.

Main Results:

  • Demonstrated that ultrasonic energy efficiently triggers clean protein desulfurization.
  • Showcased the USID strategy's mild reaction conditions and excellent functional group compatibility.
  • Successfully synthesized bioactive cyclopeptides and histone H3.5 using the USID method, even on a multi-hundred-milligram scale.

Conclusions:

  • The USID strategy provides a simple, robust, and versatile method for protein desulfurization.
  • USID is a mechanically triggered approach with potential for broad adoption in chemical protein synthesis.
  • This technique offers advantages in mildness and compatibility over existing desulfurization methods.