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Updated: May 31, 2025

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
Published on: July 7, 2014
Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform
1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
Abstract:
Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire sample, and can be compatible with different microscopy systems. This method shows great potential in connectome mapping, and might be an optimal option for future 3D multiplex immunofluorescence and RNA in situ hybridization imaging. Additional efforts would be needed to innovate labeling, imaging, and data processing strategies to fully utilize the potential of transparent embedding systems in high-resolution imaging of large-scale samples.

