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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: May 31, 2025

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Published on: July 7, 2014

Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform

Yating Yi¹, Hu Zhao²

¹State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

|January 24, 2025

Summary

Transparent embedding systems like TESOS enable consistent submicron resolution for 3D imaging. This technique holds promise for connectome mapping and advanced multiplex imaging applications.

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Last Updated: May 31, 2025

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Area of Science:

Biological Imaging
Tissue Clearing
Microscopy

Background:

Tissue clearing and 3D imaging are advancing rapidly.
Combining transparent embedding with sectioning is a key future direction.

Purpose of the Study:

To introduce and evaluate the Transparent Embedding Solvent System (TESOS).
To demonstrate its capability for submicron resolution imaging in large-scale samples.

Main Methods:

Utilized the TESOS method for transparent embedding of biological samples.
Performed imaging compatible with various microscopy systems.
Ensured consistent submicron resolution throughout entire samples.

Main Results:

TESOS provides consistent submicron resolution imaging across entire samples.
The system is compatible with diverse microscopy platforms.
Demonstrated potential for connectome mapping and multiplex imaging.

Conclusions:

TESOS represents a significant advancement in transparent embedding for 3D imaging.
It offers an optimal solution for future 3D multiplex immunofluorescence and RNA in situ hybridization.
Further innovation in labeling, imaging, and data processing is needed to maximize its potential.