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Role of Ciliary Neurotrophic Factor in Angiotensin II-Induced Hypertension

Sebastian A Potthoff1, Ivo Quack1, Yuri Mori2

  • 1Department of Nephrology, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Germany (S.A.P., I.Q., D. Arifaj, M.K., D. Argov, L.C.R., J.S.).

Hypertension (Dallas, Tex. : 1979)
|January 24, 2025
PubMed

Insights

Ciliary neurotrophic factor (CNTF) plays a key role in regulating blood pressure by modulating angiotensin II-induced responses. This cytokine impacts hypertension through a JAK2/STAT3-dependent pathway, highlighting its significance in cardiovascular regulation.

Area of Science:

  • Cardiovascular Biology
  • Immunology
  • Molecular Medicine

Background:

  • Ciliary neurotrophic factor (CNTF), a member of the IL-6 cytokine family, is primarily recognized for its neuroprotective effects.
  • The influence of CNTF on vascular function and its role in hypertension remain largely unexplored.
  • This study investigates the role of CNTF in angiotensin II (AngII)-induced hypertension.

Purpose of the Study:

  • To elucidate the role of CNTF in the development and regulation of AngII-induced hypertension.
  • To examine the impact of CNTF on vascular responses to AngII.
  • To investigate the underlying molecular mechanisms, specifically the JAK2/STAT3 pathway, involved in CNTF's effects on blood pressure.

Main Methods:

  • Utilized CNTF-knockout and wild-type mice to study AngII-induced hypertension.
  • Measured blood pressure using tail-cuff and radiotelemetry methods.
  • Assessed vascular function, renal pressor responses, and JAK2/STAT3 pathway activation in vivo, ex vivo (isolated perfused kidney), and in vitro (vascular smooth muscle cells).

Main Results:

  • CNTF-knockout mice exhibited attenuated blood pressure increases and reduced hypertensive organ damage in response to AngII compared to wild-type mice.
  • The renal pressor response to AngII was significantly decreased in CNTF-knockout mice, and this effect was partially restored by acute CNTF administration.
  • CNTF was found to augment AngII-induced JAK2/STAT3 pathway activation in vascular smooth muscle cells, a mechanism crucial for its effect on blood pressure.

Conclusions:

  • CNTF significantly impacts blood pressure regulation by modulating the AngII-induced pressor response.
  • The observed effects of CNTF are mediated through a JAK2/STAT3-dependent mechanism.
  • CNTF emerges as a critical regulatory cytokine in the context of hypertension.
Abstract

Keywords:
angiotensin IIciliary neurotrophic factorhypertensionhypertensive organ damageinterleukin-6vascular function

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