Related Experiment Video

Updated: May 31, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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Mechanism of Corylin Inhibiting the Development of Osteosarcoma: Regulating HMGB1/p38 MAPK Signaling

Rongyao Yan1, Hao Wang1, Zhenyu Cai1

  • 1Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, China.

Discovery Medicine
|January 24, 2025
PubMed
Abstract

Insights

Corylin, derived from Psoralea corylifolia L., inhibits osteosarcoma (OS) progression by suppressing High-mobility group box 1 (HMGB1) and the p38 MAPK pathway. This natural compound reduces tumor cell viability, migration, and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • High-mobility group box 1 (HMGB1) protein is implicated in osteosarcoma (OS) progression via the p38 MAPK signaling pathway.
  • Corylin, a component of Psoralea corylifolia L., exhibits anti-tumor properties.

Purpose of the Study:

  • To investigate the relationship between corylin and HMGB1 in OS.
  • To elucidate the mechanism of action of corylin on OS cells, focusing on the HMGB1-mediated p38 MAPK pathway.

Main Methods:

  • Osteosarcoma cells were treated with varying concentrations of corylin.
  • HMGB1 expression was manipulated using overexpression plasmids and siRNA.
  • Cell viability, proliferation, migration, invasion, and apoptosis were assessed.
  • Protein and mRNA levels of HMGB1, MMP-2, MMP-9, α-SMA, and p38 MAPK pathway components were analyzed via CCK-8, cell function assays, RT-qPCR, and western blotting.

Main Results:

  • Corylin reduced OS cell viability, proliferation, migration, and invasion while increasing apoptosis in a dose-dependent manner.
  • Corylin suppressed HMGB1, MMP-2, MMP-9, and α-SMA levels.
  • HMGB1 overexpression partially reversed corylin's effects, whereas HMGB1 knockdown enhanced them.

Conclusions:

  • Corylin demonstrates anti-cancer effects in osteosarcoma.
  • Corylin inhibits OS cell migration and invasion by regulating the HMGB1-mediated p38 MAPK signaling pathway.
Keywords:
corylinosteosarcomap38 MAPK signaling pathway

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