Related Experiment Video For Pb
Updated: May 31, 2025
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
Published on: September 28, 2017
The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line
Teru Kamogashira1, Shu Kikuta2, Tatsuya Yamasoba3
1Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
Background/Objectives:
This study evaluated changes in circadian clock genes and mitochondrial function in a lead (Pb)-induced toxicity model of an olfactory epithelial cell line.
Methods:
The DBC1.2 olfactory dark basal cell line was used. Dexamethasone shock was used to reset the circadian clock 24 h (Group 1) and 36 h (Group 2) after seeding. Then, 60 h after seeding, the cells were treated with or without Pb (II) nitrate in HEPES buffer for 1 h. Mitochondrial function and cell viability were evaluated 84 h after seeding.
Results:
Mitochondrial function under Pb exposure was significantly impaired in Group 1 compared with Group 2. Cell numbers and viability did not significantly differ between groups. The mitochondrial membrane potential was significantly higher in Group 1 than Group 2, both without and with Pb exposure.
Conclusions:
The circadian rhythm can alter the sensitivity to Pb-induced toxicity and mitochondrial damage in olfactory cells.
