Related Experiment Video

Updated: May 31, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

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MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma

Tae Mi Yoon1, Sun-Ae Kim1, Eun Kyung Jung1

  • 1Departments of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hwasun Hospital, Hwasun 58128, Jeonnam, Republic of Korea.

Current Oncology (Toronto, Ont.)
|January 24, 2025
PubMed

Insights

MicroRNA-129-3p (miR-129-3p) suppresses head and neck cancer progression and enhances chemoradiotherapy resistance. This microRNA was found to be downregulated in patient tumors, suggesting its therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-129 (miR-129) is implicated in cancer progression and chemoresistance.
  • miR-129-3p is the primary mature form of miR-129.
  • Its role in head and neck squamous cell carcinoma (HNSCC) progression and treatment resistance requires investigation.

Purpose of the Study:

  • To evaluate the function of miR-129-3p in HNSCC tumor progression.
  • To assess the impact of miR-129-3p on chemoradiotherapy resistance in HNSCC.
  • To determine miR-129-3p expression levels in HNSCC tissues.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-PCR) for gene expression analysis.
  • Western blotting to assess protein levels.
  • Cellular assays including proliferation, apoptosis, invasion, and migration.
  • In vivo studies using a nude mouse xenograft model.

Main Results:

  • Overexpression of miR-129-3p inhibited proliferation, invasion, and migration in HNSCC cell lines (SNU1041, SCC15, SCC25).
  • miR-129-3p induction enhanced apoptosis and sensitized HNSCC cells to radiation and cisplatin treatment.
  • Tumor tissues from HNSCC patients showed lower miR-129-3p levels compared to normal adjacent tissues.
  • In vivo, miR-129-3p overexpression significantly reduced tumor growth in a xenograft model.

Conclusions:

  • miR-129-3p acts as a tumor suppressor in HNSCC.
  • miR-129-3p enhances sensitivity to chemoradiotherapy in HNSCC.
  • These findings support the development of miR-129-3p-based therapeutic strategies for HNSCC.
Keywords:
apoptosishead and neck squamous cell carcinomamiRNAmolecular targeted therapyradioresistance

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