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A Sensitive and Selective Electrochemical Aptasensor for Carbendazim Detection.
Suthira Pushparajah1, Mahnaz Shafiei1, Aimin Yu1
1School of Science, Computing, and Engineering Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Biosensors
|January 24, 2025
Summary
We developed a sensitive electrochemical aptasensor for detecting carbendazim (CBZ) pesticide residues. This novel sensor offers rapid, selective detection of CBZ in food and environmental samples, aiding in exposure mitigation.
Area of Science:
- Analytical Chemistry
- Biosensors
- Environmental Science
Background:
- Carbendazim (CBZ) is a widely used fungicide with high environmental persistence and potential health risks.
- Accurate and rapid detection of CBZ residues is crucial for food safety and environmental monitoring.
- Aptamer-based sensors offer high specificity and sensitivity for detecting small molecules like pesticides.
Purpose of the Study:
- To develop and validate a novel electrochemical aptasensor for the sensitive and selective detection of carbendazim (CBZ).
- To establish a reliable method for monitoring CBZ in food and environmental matrices.
Main Methods:
- Fabrication of a glassy carbon electrode (GCE) modified with platinum nanoparticles (Pt NPs) and reduced graphene oxide (rGO).
- Immobilization of a CBZ-specific aptamer onto the Pt NPs via Pt-sulfur bonds.
- Electrochemical detection using K4[Fe(CN)6] as a redox probe, monitoring current changes upon CBZ binding.
Main Results:
- The aptasensor exhibited a linear response to CBZ concentrations ranging from 0.5 to 15 nM.
- Achieved a low limit of detection (LOD) of 0.41 nM for CBZ.
- Demonstrated high selectivity for CBZ over other compounds, with good recovery rates (98% in skim milk, 96% in tap water).
Conclusions:
- The developed electrochemical aptasensor is simple, sensitive, selective, and repeatable for CBZ detection.
- This sensor provides a promising platform for the effective environmental and food safety monitoring of carbendazim residues.
