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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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A Sensitive and Selective Electrochemical Aptasensor for Carbendazim Detection.

Suthira Pushparajah¹, Mahnaz Shafiei¹, Aimin Yu¹

¹School of Science, Computing, and Engineering Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.

|January 24, 2025

Summary

We developed a sensitive electrochemical aptasensor for detecting carbendazim (CBZ) pesticide residues. This novel sensor offers rapid, selective detection of CBZ in food and environmental samples, aiding in exposure mitigation.

Keywords:

carbendazim electrochemical aptasensor platinum nanoparticles reduced graphene oxide

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Area of Science:

Analytical Chemistry
Biosensors
Environmental Science

Background:

Carbendazim (CBZ) is a widely used fungicide with high environmental persistence and potential health risks.
Accurate and rapid detection of CBZ residues is crucial for food safety and environmental monitoring.
Aptamer-based sensors offer high specificity and sensitivity for detecting small molecules like pesticides.

Purpose of the Study:

To develop and validate a novel electrochemical aptasensor for the sensitive and selective detection of carbendazim (CBZ).
To establish a reliable method for monitoring CBZ in food and environmental matrices.

Main Methods:

Fabrication of a glassy carbon electrode (GCE) modified with platinum nanoparticles (Pt NPs) and reduced graphene oxide (rGO).
Immobilization of a CBZ-specific aptamer onto the Pt NPs via Pt-sulfur bonds.
Electrochemical detection using K4[Fe(CN)6] as a redox probe, monitoring current changes upon CBZ binding.

Main Results:

The aptasensor exhibited a linear response to CBZ concentrations ranging from 0.5 to 15 nM.
Achieved a low limit of detection (LOD) of 0.41 nM for CBZ.
Demonstrated high selectivity for CBZ over other compounds, with good recovery rates (98% in skim milk, 96% in tap water).

Conclusions:

The developed electrochemical aptasensor is simple, sensitive, selective, and repeatable for CBZ detection.
This sensor provides a promising platform for the effective environmental and food safety monitoring of carbendazim residues.