Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Carbon Materials in 2025: From Lightweight Structures to Sustainable and Multifunctional Platforms.

Materials (Basel, Switzerland)·2026

Same author

Modeling an SPR Sensor for Carcinoma-Related Refractive-Index Detection: The Case of CaF<sub>2</sub>/Au/Si<sub>3</sub>N<sub>4</sub>/BP Multilayer System.

Biosensors·2026

Same author

Effect of HIIT Training Modality in People with Pre-Diabetes.

Journal of functional morphology and kinesiology·2026

Same author

Phosphorus Loading Drives Microalgal Community Changes and Enhances Nutrient Removal in Photobioreactors Treating Synthetic Wastewater.

Plants (Basel, Switzerland)·2026

Same author

Structural, morphological, and optical properties of SiC/PVP nanocomposite materials by changing the SiC concentration in PVP.

RSC advances·2025

Same author

Synthesis, characterization, and photocatalytic performance of 2D/1D graphene/Ag-Ag<sub>2</sub>S hybrid nanocomposites.

Dalton transactions (Cambridge, England : 2003)·2025

Search research articles

Related Experiment Video

Updated: Apr 6, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

SPR Biosensor Based on Bilayer MoS₂ for SARS-CoV-2 Sensing.

Talia Tene¹, Stefano Bellucci², Cristian Vacacela Gomez²

¹Department of Chemistry, Universidad Técnica Particular de Loja, Loja 110160, Ecuador.

|January 24, 2025

Summary

This study developed an advanced surface plasmon resonance (SPR) biosensor using molybdenum disulfide (MoS2) and silicon nitride for highly sensitive and specific SARS-CoV-2 detection, crucial for early diagnostics.

Keywords:

MoS2 SARS-CoV-2 biosensor silicon nitride surface plasmon resonance

More Related Videos

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Related Experiment Videos

Last Updated: Apr 6, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

Nanomaterials Science
Biosensor Technology
Medical Diagnostics

Background:

The COVID-19 pandemic necessitates rapid and sensitive diagnostic tools for SARS-CoV-2.
Existing methods may lack the speed or sensitivity required for early detection.
Surface Plasmon Resonance (SPR) biosensors offer label-free detection capabilities.

Purpose of the Study:

To develop and optimize an SPR biosensor for enhanced SARS-CoV-2 detection.
To investigate the impact of advanced materials like MoS2 and silicon nitride on SPR performance.
To establish a sensitive and specific diagnostic tool for early viral detection.

Main Methods:

Systematic optimization of SPR biosensor parameters: silver layer thickness, silicon nitride dielectric layer, MoS2 layers, and ssDNA recognition layer.
Fabrication of the optimized SPR biosensor configuration.
Performance evaluation using SARS-CoV-2 detection in PBS solution, measuring sensitivity, accuracy, and quality factor.

Main Results:

The optimized SPR biosensor featured a 45 nm silver layer, 13 nm silicon nitride, 2 MoS2 layers, and a 5 nm ssDNA layer.
Achieved high sensitivity (375.01°/RIU), detection accuracy (0.002), and quality factor (38.34) at 1.0 mM SARS-CoV-2 concentration.
Demonstrated a linear response to refractive index changes, indicating quantitative analysis potential.

Conclusions:

The integration of MoS2 and silicon nitride significantly enhances SPR biosensor performance for SARS-CoV-2 detection.
The developed biosensor is a robust and precise tool for reliable SARS-CoV-2 detection, particularly for early diagnosis.
The biosensor holds potential for clinical diagnostics and epidemiological monitoring of SARS-CoV-2.