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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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Fluorogenic Biosensing with Tunable Polydiacetylene Vesicles.

John S Miller1,2, Tanner J Finney2,3, Ethan Ilagan2

  • 1Department of Materials Science and Engineering, University of California Davis, Davis, CA 95616, USA.

Biosensors
|January 24, 2025
PubMed
Summary
This summary is machine-generated.

Polydiacetylene (PDA) vesicles can be tuned for enhanced fluorogenic sensing by modifying their structure. Shorter acyl tails and specific headgroups, like ethanolamine, increase sensitivity to stimuli for improved biosensing applications.

Keywords:
biosensingfluorescencepolydiacetylenespectroscopyvesicles

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Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Nanotechnology

Background:

  • Polydiacetylenes (PDAs) are conjugated polymers exhibiting a colorimetric shift upon stimulation.
  • PDA-based sensing platforms are established for colorimetric biosensing.
  • The less-explored fluorogenic transition of PDAs offers potential for advanced biosensing.

Purpose of the Study:

  • To characterize and optimize polydiacetylene (PDA) vesicles for enhanced fluorogenic sensing.
  • To investigate how diacetylene (DA) structure influences vesicle properties and phase transitions.
  • To tune PDA vesicle sensitivity for improved biosensing applications.

Main Methods:

  • Self-assembly of diacetylene surfactant vesicles.
  • Polymerization of DA monomers within vesicles.
  • Characterization of vesicle size, stability, and polymerization kinetics.
  • Analysis of the blue-to-red phase transition and fluorescence changes upon stimuli.

Main Results:

  • Vesicle size and stability were influenced by DA hydrocarbon tail length; longer tails yielded smaller, more stable vesicles.
  • Acyl tail length and headgroup structure dictated polymerization kinetics and the blue-to-red transition.
  • Shorter acyl tails generally increased vesicle sensitivity to energetic stimuli.
  • Ethanolamine headgroups enhanced stimuli responsivity due to hydrogen bonding and backbone strain.
  • Boronic-acid headgroups resulted in unstable vesicles with poor polymerization and limited phase transition.

Conclusions:

  • PDA vesicle structure, specifically acyl tail length and headgroup, can be precisely tuned to optimize sensitivity for fluorogenic biosensing.
  • Ethanolamine headgroups show promise for developing highly responsive PDA-based sensors.
  • Understanding structure-property relationships is crucial for designing advanced PDA biosensing platforms.